Frequently Asked Questions

Please browse through our library of frequently asked questions. If you have a question that is not answered, feel free to reach us using our onlinecontact form, or you can e-mail us at info@empiregenomics.com.

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Array Comparative Genomic Hybridization FAQ

Gene Expression FAQ

BAC Screening FAQ

BAC and PAC Clones FAQ

Labeled BAC Probes for FISH FAQ

Array Comparative Genomic Hybridization FAQ


Questions	Hits
What is Array Comparative Genomic Hybridization (aCGH)?	4581
What is aCGH used for?	4582
What are the advantages of aCGH over traditional methods?	4580
What does resolution mean?	4581
What is a tiling CGH array?	4580
What are the advantages and disadvantages of BAC clone vs. Oligonucleotide arrays?	4589
What is spotted on CGH arrays?	4579
How much DNA is required for aCGH analysis?	4579
Can less than 1 ug DNA be used for aCGH analysis?	4579
How are deletions and duplications confirmed?	4580
Does Empire Genomics manufacture microarrays for aCGH?	4580
Does Empire Genomics perform Array CGH Service?	4581
What does it mean to be an Agilent Certified Services Provider?	4581
How do I get my aCGH results?	4581
How do I send in my samples for Array CGH service?	4580
Is any special paperwork required for shipping samples to you from outside the US?	243343
What is the amount and concentration of DNA required for Agilent aCGH arrays?	4581
What are the DNA QC requirements?	4580
How do I prepare the DNA sample for mailing?	4580

Gene Expression FAQ


Questions	Hits
How do I send in my samples for Gene Expression service?	4569
Is any special paperwork required for shipping sample to you from outside the US?	4571
Can I talk to someone about experimental design?	4570
How many replicate experiments should I do?	4568
Should I request a dye-flip labeling?	4568
What is the difference between a technical replicate and a biological replicate? And which type is best?	4626
How much total RNA is required for gene expression profiling?	4570
Do you offer gene expression services for low RNA yields?	4569
Why do you analyze my RNA on the Bioanalyzer?	4570
What if my total RNA sample appears to be slightly degraded on the Agilent Bioanalyzer? Can I proceed with labeling?	4571
Do you check for appropriate dye incorporation when generating labeled probes?	4569
What are the advantages and disadvantages of 1-color and 2-color hybridizations?	4571
How long will it take to get results back?	4568
What steps are taken to ensure the least amount of technical variability?	4569
What is the length of the oligos on the Agilent arrays?	4568
Are there controls spotted on the Agilent arrays?	4569
What software is used for gene expression data analysis?	4569
Where can I get more information about the Agilent platform?	4569
Who can I contact if I have questions?	4568
How do I prepare the RNA sample for mailing?	4571

BAC Screening FAQ


Questions	Hits
What is genomic library screening?	4579
What do you provide after performing BAC screening?	4568
What is an overgo probe?	4571
Why are overgos preferred over other DNA probes?	4572
Can we provide our own DNA probes?	4570
How long does this service take?	4569
Is the service guaranteed?	4570

BAC and PAC Clones FAQ


Questions	Hits
What are BAC and PAC clones?	4572
What are BAC and PAC clones used for?	4572
What clone libraries are available?	4570
How are the clones distributed?	4569
What are the vector & host details of your clones?	4570
Is there clone information available online?	4569
Can you provide more detailed DNA information about your clones?	4571
What is the expected turnaround time after I place an order?	4573
What is T1 phage?	4580
How do I purchase BAC clones?	4579
Is any special paperwork required for shipping clones outside the US?	4569
What are the shipping costs?	4569
What if the clone I purchase hybridizes to an unexpected location?	4572

Labeled BAC Probes for FISH FAQ


Questions	Hits
How much DNA comes with each labeled RP11 BAC probe?	4569
What is the turnaround time for labeled BAC probes for FISH?	4572
How do I place an order for labeled BAC probes?	4571
How many hybridizations are possible with each probe order?	4569
Is there an available protocol or instruction guide for your Custom Labeled BAC Probes?	4571
What dye colors are available for BAC probe labeling?	4573
Do you have a guarantee for the shelf life of your FISH probes?	4570
How do Empire Genomics dyes compare to dyes offered by other vendors?	4570
The FISH assay resulted in distorted chromosome morphology	4571
The FISH assay resulted in a high slide background	4570
There was a weak signal or no signal	4571
There is low signal specificity	4573
The counterstain was too bright or weak	4570
Codenaturation led to cross-hybridization	4569
The probe appears dim	4569
There is speckling due to an abnormal diffuse signal	4569
There is poor metaphase morphology	4569
Can you create custom probe panels?	4569

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