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Frequently Asked Questions

Frequently Asked Questions

Please browse through our library of frequently asked questions. If you have a question that is not answered, feel free to reach us using our onlinecontact form, or you can e-mail us at info@empiregenomics.com.

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Array Comparative Genomic Hybridization FAQ

Gene Expression FAQ

BAC Screening FAQ

BAC and PAC Clones FAQ

Labeled BAC Probes for FISH FAQ

Array Comparative Genomic Hybridization FAQ


Questions	Hits
What is Array Comparative Genomic Hybridization (aCGH)?	3634
What is aCGH used for?	3504
What are the advantages of aCGH over traditional methods?	3432
What does resolution mean?	3455
What is a tiling CGH array?	3373
What are the advantages and disadvantages of BAC clone vs. Oligonucleotide arrays?	4542
What is spotted on CGH arrays?	3426
How much DNA is required for aCGH analysis?	3445
Can less than 1 ug DNA be used for aCGH analysis?	3337
How are deletions and duplications confirmed?	3339
Does Empire Genomics manufacture microarrays for aCGH?	3323
Does Empire Genomics perform Array CGH Service?	3358
What does it mean to be an Agilent Certified Services Provider?	3329
How do I get my aCGH results?	3343
How do I send in my samples for Array CGH service?	3341
Is any special paperwork required for shipping samples to you from outside the US?	175694
What is the amount and concentration of DNA required for Agilent aCGH arrays?	3290
What are the DNA QC requirements?	3437
How do I prepare the DNA sample for mailing?	3394

Gene Expression FAQ


Questions	Hits
How do I send in my samples for Gene Expression service?	3299
Is any special paperwork required for shipping sample to you from outside the US?	3372
Can I talk to someone about experimental design?	3412
How many replicate experiments should I do?	3476
Should I request a dye-flip labeling?	3320
What is the difference between a technical replicate and a biological replicate? And which type is best?	6822
How much total RNA is required for gene expression profiling?	3370
Do you offer gene expression services for low RNA yields?	3340
Why do you analyze my RNA on the Bioanalyzer?	3445
What if my total RNA sample appears to be slightly degraded on the Agilent Bioanalyzer? Can I proceed with labeling?	3364
Do you check for appropriate dye incorporation when generating labeled probes?	3288
What are the advantages and disadvantages of 1-color and 2-color hybridizations?	3387
How long will it take to get results back?	3315
What steps are taken to ensure the least amount of technical variability?	3291
What is the length of the oligos on the Agilent arrays?	3406
Are there controls spotted on the Agilent arrays?	3308
What software is used for gene expression data analysis?	3415
Where can I get more information about the Agilent platform?	3303
Who can I contact if I have questions?	3380
How do I prepare the RNA sample for mailing?	3797

BAC Screening FAQ


Questions	Hits
What is genomic library screening?	3436
What do you provide after performing BAC screening?	3062
What is an overgo probe?	3245
Why are overgos preferred over other DNA probes?	3070
Can we provide our own DNA probes?	3101
How long does this service take?	3092
Is the service guaranteed?	3185

BAC and PAC Clones FAQ


Questions	Hits
What are BAC and PAC clones?	3213
What are BAC and PAC clones used for?	3490
What clone libraries are available?	3122
How are the clones distributed?	3141
What are the vector & host details of your clones?	3241
Is there clone information available online?	3108
Can you provide more detailed DNA information about your clones?	3173
What is the expected turnaround time after I place an order?	3170
What is T1 phage?	3617
How do I purchase BAC clones?	3203
Is any special paperwork required for shipping clones outside the US?	3105
What are the shipping costs?	3163
What if the clone I purchase hybridizes to an unexpected location?	3138
Vector pBACe3.6 Information/Map	238
At what temperature should I store my BAC clones?	132
Where can I find a Material Safety Data Sheet?	56

Labeled BAC Probes for FISH FAQ


Questions	Hits
How much DNA comes with each labeled RP11 BAC probe?	3246
What is the turnaround time for labeled BAC probes for FISH?	3398
How do I place an order for labeled BAC probes?	3109
How many hybridizations are possible with each probe order?	3163
Is there an available protocol or instruction guide for your Custom Labeled BAC Probes?	3202
What dye colors are available for BAC probe labeling?	3293
Do you have a guarantee for the shelf life of your FISH probes?	3204
How do Empire Genomics dyes compare to dyes offered by other vendors?	3125
The FISH assay resulted in distorted chromosome morphology	3155
The FISH assay resulted in a high slide background	3219
There was a weak signal or no signal	3268
There is low signal specificity	3101
The counterstain was too bright or weak	3109
Codenaturation led to cross-hybridization	3124
The probe appears dim	3054
There is speckling due to an abnormal diffuse signal	3062
There is poor metaphase morphology	3149
Can you create custom probe panels?	3233
My package arrived with no dry ice, are my labeled clones still useable?	171
Where can I find a Material Safety Data Sheet?	51