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We do not provide functional quality testing (localization, sequencing) for the RP23 mouse library. These clones are quantified for DNA amounts and immediately released from our facility. We provide no guarantee of the location of these clones in the genome nor what genes are contained within specific clones.
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The human eye visualizes the Aqua wavelength more poorly than other regions of the visible light spectrum (as below). Consequently, when choosing to use an aqua probe, it is best to use it with a target that hybridizes strongly. For example, in our own experiences we have had better success with centromere probes compared to locus probes. Our Aqua probes have been benchmarked against the leading competitors and we are as bright or brighter than they are. This material was fully QC’d and meets performance benchmarks. We offer a variety of colors for FISH probe labeling and if you want a probe with a stronger signal we would suggest you consider using green, gold, orange or red ones. We cannot guarantee the performance you will experience with the aqua dye as a results of the many variables which can effect its performance.
Empire Genomics specializes in the custom development of FISH probes based on customer specifications. Our custom FISH probe solutions extend beyond single gene, single region analysis. We offer custom labeled Fluorescence in situ Hybridization FISH Probes for a range of molecular and cytogenetic applications.
Need help finding a BAC clone that covers your region of interest? Empire Genomics offers a free web based application that allows you to search our entire BAC library database by either Clone Name, Gene Name or by Chromosome Region.
Fresh fixed paraffin embedded (FFPE) specimens require pretreatment steps prior to performing the hybridization and washing processes. These additional processing steps function to remove the paraffin embedding the tissue and break bonds which hold the cells together. Without pretreatment the DNA probes will be unable to access and bind to the target sequences.
After preparing slides for non-FFPE specimens (click here for FISH slide protocol) the next steps in the FISH preparation process are hybridization and washing. The hybridization process entails denaturation of the DNA strands to allow access and binding of the DNA probes to target sequences. The washing process removes unbound and non-specifically bound DNA probes to allow for visualization and analysis utilizing a fluorescent microscope.
The FISH probe Material Safety Data Sheet is intended to provide workers with procedures for handling or working with our FISH probes in a safe manner, and includes information such as physical data, toxicity, health effects, first aid, reactivity, storage, disposal, protective equipment, and spill-handling procedures.
Scientific Citations For FISH Probes
There are currently no articles referencing FISH probes.