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EML4-ALK Fusion FISH Probe

The EML4-ALK Fusion FISH Probe is used to confirm a fusion of the EML4 and ALK genes. The fusion of the EML4 and ALK genes has been associated with Kidney Renal Papillary Cell Carcinoma, Lung Adenocarcinoma, Lung Adenocarcinoma, Lung Adenocarcinoma, Lung Adenocarcinoma, Lung Adenocarcinoma, and Thyroid Carcinoma. These probes are FISH confirmed on normal peripheral blood in both interphase nuclei and metaphase spreads before shipment. Typical turnaround time for this product is 7-14 days after purchase.

** This product is for in vitro and research use only. This product is not intended for diagnostic use. Please note that both genes fall on the same chromosome and inter-chromosomal detection may be difficult to detect depending on the genes proximity to one another. Please consult our support staff before ordering this product to ensure that the probe can be designed to meet your specific needs.

Turnaround Time: 7-10 Business Days    Shipping Time: 1-2 Day Expedited Shipping
Download Datasheet Download FISH Protocol Download Material Safety Data Sheet

SKU Test Kits Buffer Dye Color Order Now
EML4-ALK-20-ORGR  (Standard Design) 20 (40 μL) 200 μL
EML4-ALK-20-RERE 20 (40 μL) 200 μL
EML4-ALK-20-REOR 20 (40 μL) 200 μL
EML4-ALK-20-REGO 20 (40 μL) 200 μL
EML4-ALK-20-REGR 20 (40 μL) 200 μL
EML4-ALK-20-REAQ 20 (40 μL) 200 μL
EML4-ALK-20-ORRE 20 (40 μL) 200 μL
EML4-ALK-20-OROR 20 (40 μL) 200 μL
EML4-ALK-20-ORGO 20 (40 μL) 200 μL
EML4-ALK-20-ORAQ 20 (40 μL) 200 μL
EML4-ALK-20-GORE 20 (40 μL) 200 μL
EML4-ALK-20-GOOR 20 (40 μL) 200 μL
EML4-ALK-20-GOGO 20 (40 μL) 200 μL
EML4-ALK-20-GOGR 20 (40 μL) 200 μL
EML4-ALK-20-GOAQ 20 (40 μL) 200 μL
EML4-ALK-20-GRRE 20 (40 μL) 200 μL
EML4-ALK-20-GROR 20 (40 μL) 200 μL
EML4-ALK-20-GRGO 20 (40 μL) 200 μL
EML4-ALK-20-GRGR 20 (40 μL) 200 μL
EML4-ALK-20-GRAQ 20 (40 μL) 200 μL
EML4-ALK-20-AQRE 20 (40 μL) 200 μL
EML4-ALK-20-AQOR 20 (40 μL) 200 μL
EML4-ALK-20-AQGO 20 (40 μL) 200 μL
EML4-ALK-20-AQGR 20 (40 μL) 200 μL
EML4-ALK-20-AQAQ 20 (40 μL) 200 μL

ALK Gene Summary

This gene encodes a receptor tyrosine kinase, which belongs to the insulin receptor superfamily. This protein comprises an extracellular domain, an hydrophobic stretch corresponding to a single pass transmembrane region, and an intracellular kinase domain. It plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system. This gene has been found to be rearranged, mutated, or amplified in a series of tumours including anaplastic large cell lymphomas, neuroblastoma, and non-small cell lung cancer. The chromosomal rearrangements are the most common genetic alterations in this gene, which result in creation of multiple fusion genes in tumourigenesis, including ALK (chromosome 2)/EML4 (chromosome 2), ALK/RANBP2 (chromosome 2), ALK/ATIC (chromosome 2), ALK/TFG (chromosome 3), ALK/NPM1 (chromosome 5), ALK/SQSTM1 (chromosome 5), ALK/KIF5B (chromosome 10), ALK/CLTC (chromosome 17), ALK/TPM4 (chromosome 19), and ALK/MSN (chromosome X).[provided by RefSeq, Jan 2011]

Gene Name: ALK Receptor Tyrosine Kinase

Chromosome: CHR2: 29415639 -30144477

Locus: 2p23.2-p23.1

EML4 Gene Summary

This gene is a member of the echinoderm microtubule associated protein-like family. The encoded WD-repeat protein may be involved in microtubule formation. Abnormal fusion of parts of this gene with portions of the anaplastic lymphoma receptor tyrosine kinase gene, which generates EML4-ALK fusion transcripts, is one of the primary mutations associated with non-small cell lung cancer. Alternative splicing of this gene results in two transcript variants. [provided by RefSeq, Jan 2015]

Gene Name: Echinoderm Microtubule Associated Protein Like 4

Chromosome: CHR2: 42396489 -42559688

Locus: 2p21

Gene Diseases

The EML4 ALK Fusion has been associated with the following diseases:

Disease Name
Kidney Renal Papillary Cell Carcinoma
Lung Adenocarcinoma
Lung Adenocarcinoma
Lung Adenocarcinoma
Lung Adenocarcinoma
Lung Adenocarcinoma
Thyroid Carcinoma

FISH Probe Protocols

Protocol, Procedure, or Form Name Last Modified Download

Atypical Spitzoid Neoplasms in Childhood: A Molecular and Outcome Study

Atypical spitzoid neoplasms (APNs) are primarily pediatric lesions characterized by their intermediate features; clinically and histopathologically, they fall somewhere between benign spitz nevi and malignant melanoma. The genetics of these tumors are still poorly understood. In this study, 34 APNs were analyzed using FISH and IHC. Our ALK, BRAF, and NTRK1 break-apart FISH probes were used to detect rearrangements of the genes .
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Alterations in ALK/ROS1/NTRK/MET drive a group of infantile hemispheric gliomas

While studies are plentiful on adult gliomas, infant cases are historically understudied. This team sought to account for that lack of data by analyzing glioma biopsies from 150 infants. As part of genetic profiling, our ALK break apart probe was used to detect ALK rearrangements. The team was able to divide the tumors into three genetic subtypes that were tightly tied to clinical outcome. They also found that many of the tumors harbored just a single oncogene, evidence that infant gliomas are usually single driver tumors.
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ALK-rearranged renal cell carcinomas in Polish population

Since the first report 2010, 22 cases of ALK-rearranged renal cell carcinoma (RCC) have been described. This study screened over 1000 Polish RCC patients for ALK translocations using IHC followed by FISH with our ALK break apart FISH probe. Only 31 cases were considered potentially positive or indeterminable by IHC; of these, none tested positive for ALK rearrangement with FISH. These results suggests that the mutation might be related to ethnicity, warranting further investigation into its prevalence in other ethnicities.
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