ALK Break Apart FISH Probe
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ALK Break Apart FISH Probe
Empire Genomics’ ALK Break Apart FISH Probe is designed to flank
the ALK gene and is typically used for detecting ALK rearrangements such as translocations. This probe is FISH confirmed on normal peripheral blood
metaphase spreads and interphase nuclei. The probe comes labeled in
green and orange by default, but may be
customized to meet your needs.
** This product is for in vitro and research use only. This product is not intended for diagnostic use.
Turnaround Time: 7-10 Business Days Shipping Time: 1-2 Day Expedited Shipping
This gene encodes a receptor tyrosine kinase, which belongs to the insulin receptor superfamily. This protein comprises an extracellular domain, an hydrophobic stretch corresponding to a single pass transmembrane region, and an intracellular kinase domain. It plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system. This gene has been found to be rearranged, mutated, or amplified in a series of tumours including anaplastic large cell lymphomas, neuroblastoma, and non-small cell lung cancer. The chromosomal rearrangements are the most common genetic alterations in this gene, which result in creation of multiple fusion genes in tumourigenesis, including ALK (chromosome 2)/EML4 (chromosome 2), ALK/RANBP2 (chromosome 2), ALK/ATIC (chromosome 2), ALK/TFG (chromosome 3), ALK/NPM1 (chromosome 5), ALK/SQSTM1 (chromosome 5), ALK/KIF5B (chromosome 10), ALK/CLTC (chromosome 17), ALK/TPM4 (chromosome 19), and ALK/MSN (chromosome X).[provided by RefSeq, Jan 2011]
Atypical spitzoid neoplasms (APNs) are primarily pediatric lesions characterized by their intermediate features; clinically and histopathologically, they fall somewhere between benign spitz nevi and malignant melanoma. The genetics of these tumors are still poorly understood. In this study, 34 APNs were analyzed using FISH and IHC. Our ALK, BRAF, and NTRK1 break-apart FISH probes were used to detect rearrangements of the genes .
While studies are plentiful on adult gliomas, infant cases are historically understudied. This team sought to account for that lack of data by analyzing glioma biopsies from 150 infants. As part of genetic profiling, our ALK break apart probe was used to detect ALK rearrangements. The team was able to divide the tumors into three genetic subtypes that were tightly tied to clinical outcome. They also found that many of the tumors harbored just a single oncogene, evidence that infant gliomas are usually single driver tumors.
Since the first report 2010, 22 cases of ALK-rearranged renal cell carcinoma (RCC) have been described. This study screened over 1000 Polish RCC patients for ALK translocations using IHC followed by FISH with our ALK break apart FISH probe. Only 31 cases were considered potentially positive or indeterminable by IHC; of these, none tested positive for ALK rearrangement with FISH. These results suggests that the mutation might be related to ethnicity, warranting further investigation into its prevalence in other ethnicities.