Catalog and Custom FISH Probes

Custom FISH Probes

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The Human Genome contains numerous areas of cross-homology that can result in multiple loci signals in FISH probes. When selecting RP11 or RP23 Probes to use as FISH probes, we strongly suggest that you reference the eFISH tool made available by The Centre for Applied Genomics to determine if your probe of interest demonstrates any probability of regions of cross-hybridization in the genome. If cross-hybridization is suggested, this can often be removed or decreased by increasing the stringency of your washes. In areas of high cross-homology, selecting a probe upstream or downstream of the desired clone with less cross-homology is recommended.

Following these recommendations, while not guaranteed to remove cross-hybridizations, can help to troubleshoot and better design probes to work for your needs.



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